Ultra Performance Liquid Chromatography : an Introduction and Review
نویسندگان
چکیده
Ultra Performance Liquid Chromatography (UPLC) is a relatively new technique giving new possibilities in liquid chromatography, especially concerning decrease of time and solvent consumption. UPLC chromatographic system is designed in a special way to withstand high system back-pressures. The quality control analyses of various pharmaceutical formulations are transferred from HPLC to UPLC system. The separation on UPLC is performed under very high pressures (up to 100 MPa) but it has no negative influence on analytical column or other components of chromatographic system. Separation efficiency remains maintained or is even improved by UPLC. This review introduces the theory of UPLC, and summarizes some of the most recent work in the field.
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